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Test – I : Sperm pH
To determine Semen pH
Test – II : Membrane integrity of Sperms Physical Intactness of Sperm
Evaluation of physical intactness of sperm membrane by supra vital staining. This test provides estimation of motility with good internal control. The sum of dead spermatozoa (i.e. Stained) and motile spermatozoa (i.e. Unstained) should not be more than 100%
Test – III : Morphology Stain
Evaluate defects of Head, Neck & Tail of sperm.